Browse Skills — Page 16

alterlab-blast

AlterLab-IEU/AlterLab-Academic-Skills

Runs NCBI BLAST+ 2.17.0 sequence searches from the command line: makeblastdb (with -parse_seqids), blastn/blastp/blastx/tblastn with tabular -outfmt 6/7 for parsing, correct -task choice (megablast vs blastn vs blastn-short), -taxids/-negative_taxids taxonomic scoping, and -mt_mode multithreading; plus a DIAMOND blastp --ultra-sensitive path for large protein searches. Warns that -max_target_seqs is a heuristic keep-count, not a top-N best-hits filter. Use when the user wants command-line BLAST, makeblastdb, a local BLAST database, blastn/blastp/blastx/tblastn searches, or DIAMOND protein search. For the Bio.Blast web NCBIWWW API prefer alterlab-biopython; for quick one-liner database lookups prefer alterlab-gget. Part of the AlterLab Academic Skills suite.

alterlab-boltz

AlterLab-IEU/AlterLab-Academic-Skills

Co-fold biomolecular complexes with Boltz-2, an open AlphaFold3-style model — predict protein + ligand (SMILES/CCD), protein + nucleic-acid, and multi-chain structures in one pass, with binding-affinity prediction. Use when folding a protein together with a small-molecule ligand, predicting a holo (ligand-bound) complex or its binding affinity, or co-folding protein–DNA/RNA assemblies. For protein-only or protein–protein folding without ligands prefer alterlab-alphafold; for antibody–antigen complexes prefer alterlab-chai; to dock a ligand into a FIXED receptor structure prefer alterlab-diffdock; to look up an existing structure prefer alterlab-pdb. Part of the AlterLab Academic Skills suite.

alterlab-borzoi

AlterLab-IEU/AlterLab-Academic-Skills

Predict genome-wide functional genomics tracks from DNA sequence with Borzoi (Linder 2025) — a sequence-to-function model outputting RNA-seq, CAGE, ATAC, and ChIP coverage across long context, used to score non-coding and regulatory variant effects. Use when predicting functional tracks from a DNA sequence, scoring a non-coding/regulatory variant's effect on expression or chromatin, or doing in-silico mutagenesis of a locus. To LOOK UP a variant's population frequency prefer alterlab-gnomad; for its clinical significance prefer alterlab-clinvar; for protein-structure effects prefer alterlab-alphafold; for single-cell foundation models prefer alterlab-scgpt. Part of the AlterLab Academic Skills suite.

alterlab-brenda

AlterLab-IEU/AlterLab-Academic-Skills

Access the BRENDA enzyme database via its SOAP API to retrieve kinetic parameters (Km, kcat, Ki), reaction equations, organism data, and substrate-specific enzyme information indexed by EC number. Use when looking up enzyme kinetics, turnover numbers, or substrate specificity for biochemical research and metabolic pathway analysis. Part of the AlterLab Academic Skills suite.

alterlab-causal-inference

AlterLab-IEU/AlterLab-Academic-Skills

Estimates causal effects from observational and quasi-experimental data — difference-in-differences, instrumental variables, regression discontinuity, panel fixed effects, propensity-score / doubly-robust methods, and heterogeneous treatment effects (CATE) — using the verified Python stack: statsmodels and linearmodels (PanelOLS, IV2SLS), pyfixest (feols, event studies, Sun-Abraham, did2s), DoWhy (identify -> estimate -> refute), EconML (LinearDML, CausalForestDML, DRLearner), and rdrobust for RD. It names the identifying assumption before estimating and runs a refutation/robustness check after. Use when the request mentions difference-in-differences, instrumental variables, regression discontinuity, fixed effects / panel causal estimation, propensity scores, or treatment-effect estimation from non-randomized data. For choosing the design first prefer alterlab-ssci-design-gate; for plain regression or descriptive stats prefer alterlab-statistical-analysis. Part of the AlterLab Academic Skills suite.

alterlab-cbioportal

AlterLab-IEU/AlterLab-Academic-Skills

Query cBioPortal via its keyless REST API for cancer genomics across TCGA, GENIE, MSK-IMPACT and hundreds of studies — somatic mutations, copy-number alterations (GISTIC), mRNA/protein expression, structural variants, and patient-level clinical/survival data. Use when asked how often a gene is mutated/amplified/deleted in a tumor type, to profile oncogenes or tumor suppressors across cancers (pan-cancer alteration frequency), to pull patient-level mutations joined to OS/clinical outcomes, or to validate a cancer target from cohort genomics. For germline variant pathogenicity use alterlab-clinvar; for mutational-signature (SBS) decomposition use alterlab-cosmic; for CRISPR/RNAi gene-dependency use alterlab-depmap; for aggregated target-disease evidence use alterlab-opentargets. Part of the AlterLab Academic Skills suite.

alterlab-cellxgene

AlterLab-IEU/AlterLab-Academic-Skills

Query the CZ CELLxGENE Census (61M+ cells) programmatically via cellxgene-census and TileDB-SOMA, slicing expression by tissue, disease, or cell type and returning AnnData. Use when pulling reference single-cell RNA-seq data from the largest curated public atlas, running population-scale queries, or benchmarking your data against a reference — for analyzing your own dataset use scanpy or scvi-tools. Part of the AlterLab Academic Skills suite.

alterlab-chai

AlterLab-IEU/AlterLab-Academic-Skills

Predict biomolecular complexes with Chai-1, an open AlphaFold3-style model that folds multi-entity assemblies (proteins, ligands, nucleic acids) from a single typed FASTA — strong on antibody–antigen and protein–ligand complexes, with optional MSA and restraint inputs. Use when predicting an antibody–antigen complex, folding a mixed protein/ligand/nucleic-acid assembly described in one FASTA, or generating a complex with experimental restraints. For binding-affinity prediction or a ligand-focused co-fold prefer alterlab-boltz; for protein-only or protein–protein folding prefer alterlab-alphafold; to dock into a fixed receptor prefer alterlab-diffdock. Part of the AlterLab Academic Skills suite.

alterlab-chembl

AlterLab-IEU/AlterLab-Academic-Skills

Query ChEMBL via the chembl_webresource_client Python client for curated bioactive molecules and drug-like compound libraries at scale — search compounds by structure or physicochemical properties, retrieve bioactivity measurements (IC50, Ki, EC50), and find inhibitors of a target. Use when screening chemical libraries, mining curated bioactivity for a protein, running SAR studies, or sourcing medicinal-chemistry data; for measured protein-ligand binding affinities (Ki/Kd/IC50) prefer alterlab-bindingdb instead. Part of the AlterLab Academic Skills suite.

alterlab-cirq

AlterLab-IEU/AlterLab-Academic-Skills

Builds, simulates, and runs quantum circuits with Cirq, Google Quantum AI's framework for NISQ hardware, noise-aware low-level circuit design, and noise characterization. Use when targeting Google Quantum AI processors (Sycamore/Weber), designing noise-aware NISQ circuits, or running characterization experiments (randomized benchmarking, XEB). For IBM Quantum hardware and Qiskit Runtime prefer alterlab-qiskit; for gradient-trained quantum ML and hybrid quantum-classical models prefer alterlab-pennylane; for open-system Lindblad/master-equation dynamics prefer alterlab-qutip. Part of the AlterLab Academic Skills suite.

alterlab-citation-graph

AlterLab-IEU/AlterLab-Academic-Skills

Free, key-less ResearchRabbit analog — builds a citation and co-citation graph around one or more seed papers using the OpenAlex API. Walks both directions of the citation network (works the seed cites and works that cite the seed), ranks the discovered neighbourhood by co-citation strength and bibliographic coupling to surface the papers most central to a topic's literature, and exports the network as GraphML (Gephi / Cytoscape / yEd) and JSON. Use when mapping a literature landscape, finding seminal or highly co-cited papers from a seed DOI, snowballing a reference network, building a citation map / co-citation analysis, or visualizing how a research area's papers connect — no API key required (polite mailto only). Part of the AlterLab Academic Skills suite.

alterlab-citation-mgmt

AlterLab-IEU/AlterLab-Academic-Skills

Manages citations for academic research — searches Google Scholar and PubMed for papers, extracts accurate metadata, validates citations, and generates properly formatted BibTeX entries. Use when finding papers, verifying citation information, converting DOIs to BibTeX, checking reference accuracy in scientific writing, or building a bibliography. Part of the AlterLab Academic Skills suite.

alterlab-citation-verifier

AlterLab-IEU/AlterLab-Academic-Skills

Verifies that every entry in a bibliography ACTUALLY EXISTS by cross-checking it against four keyless public scholarly APIs (Crossref, OpenAlex, Semantic Scholar, arXiv) with a polite mailto identifier, resolving DOI/arXiv IDs, fuzzy-matching title and authors (difflib SequenceMatcher ratio >=0.70), flagging retractions marked in Crossref (update-to) or OpenAlex (is_retracted), and emitting per-entry JSON verdicts mapped to the AlterLab citation-hallucination taxonomy (TF/PAC/IH/PH/SH). Accepts BibTeX, a DOI/arXiv ID list, or free-form references; degrades gracefully offline by emitting 'unverified' verdicts and never silently passing. Use when the request mentions verify citations, check references, fabricated or hallucinated references, fake DOI, retraction check, bibliography audit, or reference existence check. Does NOT write or draft papers — for authoring a manuscript (whose citation-check mode inserts citations) prefer alterlab-paper-writer instead. Part of the AlterLab Academic Skills suite.

alterlab-clinical-decision

AlterLab-IEU/AlterLab-Academic-Skills

Generates professional clinical decision support (CDS) documents for pharmaceutical and clinical research settings — biomarker-stratified patient cohort analyses with outcomes and evidence-based treatment recommendation reports with decision algorithms, supporting GRADE evidence grading, statistical analysis (hazard ratios, survival curves, waterfall plots), biomarker integration, and regulatory compliance, output as publication-ready LaTeX/PDF. Use when building a CDS document, cohort analysis, or treatment recommendation report for drug development, clinical research, or evidence synthesis, or when GRADE grading, hazard ratios, survival/waterfall plots, or biomarker stratification are requested. Part of the AlterLab Academic Skills suite.

alterlab-clinical-reports

AlterLab-IEU/AlterLab-Academic-Skills

Writes comprehensive clinical reports — case reports (CARE guidelines), diagnostic reports (radiology, pathology, lab), clinical trial reports (ICH-E3, SAE, CSR), and patient documentation (SOAP notes, H&P, discharge summaries) — with templates, regulatory compliance (HIPAA, FDA, ICH-GCP), and validation tools. Use when drafting a case report for journal publication, a radiology/pathology/lab diagnostic report, an ICH-E3 clinical study report (CSR) or SAE narrative, or SOAP/H&P/discharge patient records needing regulatory-compliant formatting. Part of the AlterLab Academic Skills suite.

alterlab-clinicaltrials

AlterLab-IEU/AlterLab-Academic-Skills

Query ClinicalTrials.gov via its API v2 to search trials by condition, drug, location, recruitment status, or phase and retrieve trial details by NCT ID. Use when finding interventional or observational studies, checking trial status and eligibility for patient matching, or exporting clinical trial records for research. Part of the AlterLab Academic Skills suite.

alterlab-clinpgx

AlterLab-IEU/AlterLab-Academic-Skills

Access ClinPGx pharmacogenomics data (the successor to PharmGKB) to query gene-drug interactions, CPIC/DPWG dosing guidelines, drug labels, and pharmacogene records. Use when interpreting pharmacogenes (CYP2D6, CYP2C19, TPMT, DPYD, SLCO1B1), looking up genotype-guided drug dosing, checking PGx drug-safety associations (e.g. HLA-B*57:01 and abacavir), or supporting precision medicine and clinical pharmacogenomics decisions. For star-allele definitions/frequencies see PharmVar; for germline/somatic variant pathogenicity see alterlab-clinvar. Part of the AlterLab Academic Skills suite.

alterlab-clinvar

AlterLab-IEU/AlterLab-Academic-Skills

Query NCBI ClinVar via the E-utilities API or FTP for the clinical significance (pathogenicity) of human germline genetic variants, searching by gene, variant, condition, or genomic position and interpreting ACMG/AMP classifications and review-status star ratings. Use when assessing whether a variant is pathogenic, likely pathogenic, VUS, likely benign, or benign, resolving conflicting interpretations, or annotating a VCF with ClinVar clinical significance. For population allele frequencies by ancestry use alterlab-gnomad; for somatic cancer mutation frequencies use alterlab-cosmic. Part of the AlterLab Academic Skills suite.

alterlab-cobrapy

AlterLab-IEU/AlterLab-Academic-Skills

Build and analyze genome-scale constraint-based metabolic models with COBRApy — flux balance analysis (FBA), flux variability analysis (FVA), gene and reaction knockouts, flux sampling, and SBML model I/O. Use when simulating metabolic networks, predicting growth or knockout phenotypes, or running systems-biology and metabolic-engineering analyses on SBML genome-scale models. Part of the AlterLab Academic Skills suite.

alterlab-cosmic

AlterLab-IEU/AlterLab-Academic-Skills

Access the COSMIC catalogue of somatic mutations in cancer to query somatic mutations, the Cancer Gene Census, mutational signatures, and gene fusions (authentication required). Use when curating known cancer driver genes, looking up recurrent somatic mutations in a gene, or interpreting mutational signatures for cancer research and precision oncology. Not for germline pathogenicity calls (use alterlab-clinvar) or interactive cohort visualization like OncoPrints and survival from study data (use alterlab-cbioportal). Part of the AlterLab Academic Skills suite.

alterlab-dask

AlterLab-IEU/AlterLab-Academic-Skills

Scales pandas/NumPy workflows beyond memory with Dask distributed computing — parallel DataFrames, arrays, delayed task graphs, and cluster execution. Use when existing pandas/NumPy code must run on larger-than-RAM data or across clusters, for parallel file processing, distributed ML, or integration with existing pandas code. For out-of-core analytics on a single machine prefer vaex; for in-memory speed prefer polars. Part of the AlterLab Academic Skills suite.

alterlab-datacommons

AlterLab-IEU/AlterLab-Academic-Skills

Query Google Data Commons for public statistical data aggregated from global sources, resolving geographic entities and pulling time-series statistics. Use when working with demographic data, economic indicators, health statistics, or environmental data — population counts, GDP figures, unemployment rates, disease prevalence — or when resolving places to DCIDs and exploring relationships between statistical entities. Part of the AlterLab Academic Skills suite.

alterlab-datamol

AlterLab-IEU/AlterLab-Academic-Skills

Wraps RDKit in a high-level, pandas-friendly datamol interface with sensible defaults for everyday drug discovery — SMILES/SDF loading into DataFrames, molecule standardization, descriptors, fingerprints, Butina clustering, 3D conformer generation, scaffold analysis, and parallel batch processing, returning native rdkit.Chem.Mol objects. Use when running standard cheminformatics pipelines on molecule tables with minimal boilerplate; for low-level control, custom sanitization, or specialized algorithms prefer alterlab-rdkit. Part of the AlterLab Academic Skills suite.

alterlab-deep-research

AlterLab-IEU/AlterLab-Academic-Skills

Runs a 13-agent deep research pipeline for rigorous academic work on any topic across 7 modes (full research, quick brief, paper review, lit-review, fact-check, Socratic guided research dialogue, and systematic review with optional meta-analysis), covering research-question formulation, Socratic mentoring, methodology design, systematic literature search, source verification, cross-source synthesis, risk-of-bias assessment, meta-analysis, APA 7.0 report compilation, editorial and devil's-advocate review, ethics review, and post-research literature monitoring. Use when the request mentions research, deep research, literature review, systematic review, meta-analysis, PRISMA, evidence synthesis, fact-check, guide my research, help me think through, or 研究, 深度研究, 文獻回顧, 文獻探討, 系統性回顧, 後設分析, 事實查核, 引導我的研究, 幫我釐清, 幫我想想, 我不確定要研究什麼, 研究方向, 研究主題. Part of the AlterLab Academic Skills suite.

alterlab-deepchem

AlterLab-IEU/AlterLab-Academic-Skills

Runs molecular machine learning with DeepChem — diverse featurizers, pre-built MoleculeNet benchmark datasets, and pre-trained models (ChemBERTa, GROVER) for property prediction (ADMET, toxicity, solubility) via traditional ML or graph neural networks. Use when running end-to-end molecular ML experiments that need MoleculeNet benchmarks, scaffold splitting, or ready-made models with minimal setup; for building custom PyTorch graph architectures prefer alterlab-torchdrug, and for standalone molecule-to-feature-vector generation prefer alterlab-molfeat. Part of the AlterLab Academic Skills suite.

alterlab-deeptools

AlterLab-IEU/AlterLab-Academic-Skills

Process and visualize deep-sequencing coverage with the deepTools CLI — convert BAM to bigWig (bamCoverage), build log2 ratio tracks (bamCompare), run QC (multiBamSummary correlation, PCA, plotFingerprint), apply the ATAC-seq Tn5 shift (alignmentSieve --ATACshift), and make TSS/peak heatmaps and profiles (computeMatrix, plotHeatmap, plotProfile). Use for coverage tracks, signal heatmaps/profiles, normalization (RPGC/CPM/RPKM), and effective-genome-size lookups for ChIP-seq, ATAC-seq, MNase-seq, or RNA-seq. NOT for per-read/CIGAR/MAPQ BAM record access — that is pysam. Part of the AlterLab Academic Skills suite.

alterlab-denario

AlterLab-IEU/AlterLab-Academic-Skills

Runs Denario (AstroPilot-AI), a multiagent AI system for scientific research assistance that automates end-to-end research workflows from a described dataset through idea, methodology, computational results, and a publication-ready LaTeX paper. Built on AG2 + LangGraph with a cmbagent analysis backend. Use when driving the Denario pipeline (Denario.get_idea/get_method/get_results/get_paper), generating research ideas from a dataset description, auto-developing methodology, executing analysis agents, or emitting a journal-formatted (APS/AAS/JHEP/ICML/NeurIPS/PASJ) LaTeX manuscript. Part of the AlterLab Academic Skills suite.

alterlab-depmap

AlterLab-IEU/AlterLab-Academic-Skills

Query the Cancer Dependency Map (DepMap) for cancer cell line gene dependency scores (CRISPR Chronos), drug sensitivity data, and gene effect profiles. Use when identifying cancer-specific genetic vulnerabilities, finding synthetic lethal interactions, checking whether a gene is essential in given cell lines, or validating oncology drug targets. Part of the AlterLab Academic Skills suite.

alterlab-dergipark

AlterLab-IEU/AlterLab-Academic-Skills

Harvests article metadata, abstracts, and full-text PDFs from DergiPark (TÜBİTAK ULAKBİM's national journal-hosting platform, ~2,537 journals) via its verified platform-wide OAI-PMH endpoint (https://dergipark.org.tr/api/public/oai/; verbs Identify/ListSets/ListRecords/GetRecord; prefixes oai_dc/oai_mods/oai_marc/oai_etdms; setSpec=journal-slug), parses Highwire citation_* and DC.* meta tags on /pub/{slug}/article/{id} pages, pulls PDFs from the citation_pdf_url path, and emits BibTeX/RIS locally. Use when the user wants to harvest a Turkish journal, fetch DergiPark articles, list a journal archive, get BibTeX/RIS for a DergiPark paper, or read a journal aim-and-scope (öz/kapsam). For TR Dizin indexing status use alterlab-trdizin; for YÖK theses use alterlab-yok-tez; for academic profiles use alterlab-yok-akademik. Part of the AlterLab Academic Skills suite.

alterlab-diffdock

AlterLab-IEU/AlterLab-Academic-Skills

Predicts protein-ligand binding poses with DiffDock diffusion-based molecular docking from PDB structures and SMILES, producing pose confidence scores for virtual screening and structure-based drug design. Use when docking ligands into a protein, generating binding poses, or screening compounds against a target; not for binding affinity prediction. Part of the AlterLab Academic Skills suite.

alterlab-digital-humanities

AlterLab-IEU/AlterLab-Academic-Skills

Applies computational methods to humanities research — text mining and NLP (LDA/BERTopic topic modeling, sentiment, named entity recognition with spaCy/NLTK), corpus linguistics (concordance, collocation, keyness), digital archives (Dublin Core, TEI XML), GIS for history, network analysis, stylometry and authorship attribution, OCR (Tesseract, Kraken, Transkribus), and data visualization (Gephi, Palladio). Use when distant-reading a literary corpus, mapping historical events or trade networks, attributing disputed authorship, digitizing historical documents, or building digital scholarly editions. Part of the AlterLab Academic Skills suite.

alterlab-dnanexus

AlterLab-IEU/AlterLab-Academic-Skills

Develops and runs genomics pipelines on the DNAnexus cloud platform using the dxpy Python SDK and dx CLI — build apps/applets, write dxapp.json, upload/download data, and execute jobs/workflows over FASTQ/BAM/VCF files. Use when building or running a DNAnexus app, applet, or workflow, writing dxapp.json, using dx-app-wizard or dx build, calling dxpy (find_data_objects, DXApplet.run, upload_local_file), or uploading/downloading sequencing data on DNAnexus. For LatchBio (Latch SDK @workflow/@task, LatchFile) use alterlab-latchbio instead; for Benchling LIMS use alterlab-benchling. Part of the AlterLab Academic Skills suite.

alterlab-docentlik-eligibility

AlterLab-IEU/AlterLab-Academic-Skills

Runs a PARTIAL pre-screen of a Turkish associate-professorship (doçentlik) publication list against the ÜAK (Üniversitelerarası Kurul) Sağlık Bilimleri TABLO 10 criteria, applying the author-share rule (full; 0.8 + 0.5; lead-author half-the-rest split) and pass-fail checking the four computable minimums (≥100 total, ≥90 post-doctorate, ≥40 SCIE/SSCI article points, ≥3 lead-author Q1–Q4 articles). It deliberately does NOT emit an ELIGIBLE verdict — it lists the unmodelled mandatory minimums (TR Dizin/national article, citation, congress, teaching, per-category caps) for the user to verify. Use when the user wants to estimate doçentlik eligibility, calculate ÜAK points, or audit lead-author (başlıca yazar) requirements; resolve a journal's live TR Dizin status with alterlab-trdizin first, and compute the akademik teşvik (academic-incentive) score with alterlab-akademik-tesvik. Always verify the full live TABLO 10 — criteria change each term and differ per field. Part of the AlterLab Academic Skills suite.

alterlab-drugbank

AlterLab-IEU/AlterLab-Academic-Skills

Access and analyze drug information from the DrugBank database — drug properties, interactions, targets, pathways, chemical structures, and pharmacology data. Use when working with pharmaceutical data, drug discovery research, drug-drug interaction analysis, target identification, chemical similarity searches, ADMET predictions, or any task needing detailed drug and drug-target records from DrugBank. Part of the AlterLab Academic Skills suite.

alterlab-eda

AlterLab-IEU/AlterLab-Academic-Skills

Exploratory data analysis (EDA) on a scientific data file — auto-detects the format, runs structure/quality/statistics checks, and writes a markdown EDA report with downstream recommendations. Use when asked to "explore", "analyze", "summarize", "profile", or "QC" a data file, or to understand its structure/content/quality before deciding what analysis to run. Covers tabular (.csv .tsv .xlsx .parquet), arrays (.npy .npz .hdf5 .h5 .mat .fits), sequence/genomics (.fasta .fastq .sam .bam .vcf .bed .gff .gtf .h5ad), microscopy (.tif .nd2 .czi .lif .ims .dcm .nii), spectroscopy/MS (.mzML .mzXML .mgf .fid .jdx), chemistry (.pdb .cif .mol .sdf .xyz .gro), and proteomics/metabolomics (.pepXML .mzid .mzTab). For zero-shot forecasting of a series use alterlab-timesfm; to create/configure a chunked cloud array store use alterlab-zarr. Part of the AlterLab Academic Skills suite.

alterlab-edgartools

AlterLab-IEU/AlterLab-Academic-Skills

Accesses, analyzes, and extracts data from SEC EDGAR filings using the edgartools Python library. Use when working with SEC filings, financial statements (income statement, balance sheet, cash flow), XBRL financial data, insider trading (Form 4), institutional holdings (13F), company financials, annual/quarterly reports (10-K, 10-Q), proxy statements (DEF 14A), 8-K current events, company screening by ticker/CIK/industry, multi-period financial analysis, or any SEC regulatory filings. Part of the AlterLab Academic Skills suite.

alterlab-ena

AlterLab-IEU/AlterLab-Academic-Skills

Access the European Nucleotide Archive (ENA) via its API and FTP to retrieve DNA/RNA sequences, raw sequencing reads (FASTQ), and genome assemblies by accession, with support for multiple formats. Use when downloading reads or sequences for a study, run, or sample accession, or when sourcing nucleotide data for genomics and bioinformatics pipelines. Part of the AlterLab Academic Skills suite.

alterlab-ensembl

AlterLab-IEU/AlterLab-Academic-Skills

Query the Ensembl genome database REST API across 250+ species for gene lookups, sequence retrieval, variant analysis, comparative genomics, orthologs, and Variant Effect Predictor (VEP) annotations. Use when mapping gene IDs or coordinates, fetching genomic sequence, finding orthologs across species, or predicting variant consequences for genomic research. Part of the AlterLab Academic Skills suite.

alterlab-esm

AlterLab-IEU/AlterLab-Academic-Skills

Run ESM protein language models — ESM3 for generative multimodal protein design across sequence, structure, and function, and ESM C for efficient embeddings and representations — locally or via the cloud Forge API. Use when working with protein sequences, structures, or function prediction, designing novel proteins, generating protein embeddings, performing inverse folding, or doing protein-engineering tasks. Part of the AlterLab Academic Skills suite.

alterlab-etetoolkit

AlterLab-IEU/AlterLab-Academic-Skills

Manipulate, annotate, and render phylogenetic trees programmatically with the ETE Toolkit (ete3) — parse and edit Newick/NHX, detect duplication/speciation events, infer orthology and paralogy, query NCBI taxonomy, and export PDF/SVG figures. Use when traversing or reformatting tree files, doing phylogenomic comparative analysis, or producing publication tree graphics in Python. Part of the AlterLab Academic Skills suite.

alterlab-fda

AlterLab-IEU/AlterLab-Academic-Skills

Query the openFDA API for drugs, medical devices, adverse event reports, recalls, regulatory submissions (510k, PMA), and substance identification (UNII). Use when searching FDA safety data, pharmacovigilance and adverse-event signals, device clearances, drug labels, or recall records for regulatory data analysis and safety research. Part of the AlterLab Academic Skills suite.

alterlab-figure-qa

AlterLab-IEU/AlterLab-Academic-Skills

Verify publication figures with a render-then-check QA pass — data-fidelity against every underlying row, axis/label floor-and-ceiling legibility, bounding-box collision detection for overlapping text/markers, and 300-dpi print-readiness. Use when proofing or auditing a finished figure for correctness and print quality, catching mislabeled or overlapping elements, or confirming a plot faithfully represents its data before submission. For CREATING the plot prefer alterlab-matplotlib (or alterlab-seaborn / alterlab-plotly); for multi-panel publication layout prefer alterlab-scientific-viz; for schematic diagrams prefer alterlab-scientific-schematics. Part of the AlterLab Academic Skills suite.

alterlab-flowio

AlterLab-IEU/AlterLab-Academic-Skills

Parse and write FCS (Flow Cytometry Standard) files v2.0-3.1 with FlowIO — extract event data as NumPy arrays, read $-keyword metadata and channel/parameter definitions, and convert events to CSV or pandas DataFrame. Use when loading raw .fcs flow-cytometry files, inspecting channels and metadata, or preprocessing cytometry data for downstream gating and analysis. Part of the AlterLab Academic Skills suite.

alterlab-fluidsim

AlterLab-IEU/AlterLab-Academic-Skills

Runs computational fluid dynamics simulations with the FluidSim Python framework using pseudospectral FFT methods, with HPC support and output analysis. Use when simulating Navier-Stokes equations (2D/3D), shallow water equations, or stratified flows, or when analyzing turbulence, vortex dynamics, or geophysical flows. Part of the AlterLab Academic Skills suite.

alterlab-fred

AlterLab-IEU/AlterLab-Academic-Skills

Queries the FRED (Federal Reserve Economic Data) API for 800,000+ economic time series from 100+ sources, covering GDP, unemployment, inflation, interest rates, exchange rates, housing, and regional data. Use for macroeconomic analysis, financial research, policy studies, economic forecasting, fetching U.S. or international economic indicators by FRED series ID, and academic research requiring historical economic time series. Part of the AlterLab Academic Skills suite.

alterlab-gene-db

AlterLab-IEU/AlterLab-Academic-Skills

Query NCBI Gene via the E-utilities and Datasets APIs, searching by gene symbol or Gene ID and retrieving gene information (RefSeqs, GO terms, genomic locations, associated phenotypes) including batch lookups. Use when resolving gene symbols to IDs, annotating gene lists, or pulling functional and positional gene metadata for downstream analysis. Part of the AlterLab Academic Skills suite.

alterlab-generate-image

AlterLab-IEU/AlterLab-Academic-Skills

Generates or edits raster images via AI models (FLUX.2, Gemini 3.1 Flash Image / "Nano Banana 2") through an OpenRouter API key. Use when the request is to generate or edit a photo, illustration, artwork, concept art, poster hero image, or presentation/slide visual asset — anything that is not a technical diagram or a data chart. For flowcharts, circuits, pathways, neural-net architectures, and technical/methodology diagrams use alterlab-scientific-schematics; for plotting numeric data (scatter, bar, line) use alterlab-matplotlib instead. Part of the AlterLab Academic Skills suite.

alterlab-geniml

AlterLab-IEU/AlterLab-Academic-Skills

Machine learning on genomic interval data (BED files) with the geniml Python package — region embeddings (Region2Vec), joint region+metadata embeddings (BEDspace/StarSpace), single-cell ATAC-seq embeddings (scEmbed), consensus peak sets / universes (build-universe), tokenization, BEDshift randomization, and BBClient/BEDbase caching. Use when training or using region/cell embeddings, clustering scATAC-seq, building a tokenization universe from BED collections, or any ML/feature-learning task over genomic regions. NOT for plain interval arithmetic (overlap/intersect/merge counts) — that is gtars, not geniml. Part of the AlterLab Academic Skills suite.

alterlab-geo

AlterLab-IEU/AlterLab-Academic-Skills

Access NCBI GEO (Gene Expression Omnibus) for gene expression and functional genomics data — search and download microarray and RNA-seq datasets by GSE, GSM, GPL, or GDS accession and retrieve SOFT, MINiML, and series matrix files. Use when locating public expression datasets, fetching processed expression matrices, downloading a study's supplementary files, or sourcing per-study transcriptomics data for differential-expression analysis. For raw FASTQ sequencing reads by SRA/ENA run accession use alterlab-ena; for reference tissue-expression baselines (median TPM across human tissues) use alterlab-gtex; for cancer cohort somatic mutations and copy-number use alterlab-cbioportal. Part of the AlterLab Academic Skills suite.

alterlab-geomaster

AlterLab-IEU/AlterLab-Academic-Skills

Covers geospatial science across remote sensing, GIS, spatial analysis, and machine learning for earth observation — satellite imagery processing (Sentinel, Landsat, MODIS, SAR, hyperspectral), raster and DEM operations, spectral indices (NDVI/EVI/NDWI), spatial statistics, point cloud processing, network analysis, and cloud-native workflows (STAC, COG, Planetary Computer), with examples across Python, R, Julia, JavaScript, C++, Java, Go, and Rust. Use for remote sensing workflows, satellite/raster image classification, terrain/slope/hillshade analysis, spatial ML on earth-observation data, hydrological modeling, marine spatial analysis, or atmospheric science. For pure tabular vector work with no raster/EO aspect (plain GeoPandas sjoin, buffer, overlay, dissolve, choropleths) prefer the geopandas skill; for celestial-sphere astronomy coordinates (ICRS/galactic, FITS, WCS) prefer the astropy skill. Part of the AlterLab Academic Skills suite.

alterlab-geopandas

AlterLab-IEU/AlterLab-Academic-Skills

Reads, writes, and analyzes geospatial vector data with the GeoPandas Python library (shapefiles, GeoJSON, GeoPackage), with PostGIS support and integration with matplotlib, folium, and cartopy. Use for spatial analysis and geometric operations — buffer analysis, spatial joins and overlays between datasets, dissolving boundaries, clipping, calculating areas and distances, reprojecting coordinate systems, choropleth mapping, or converting between vector file formats. This is for tabular vector data; for raster/satellite/DEM work, spectral indices (NDVI), or spatial ML on earth observation prefer the geomaster skill. Part of the AlterLab Academic Skills suite.

alterlab-gget

AlterLab-IEU/AlterLab-Academic-Skills

Run fast one-liner queries to 20+ bioinformatics databases from the gget CLI or Python — gene info (Ensembl), BLAST, AlphaFold structures, Enrichr enrichment, and more. Use for quick interactive lookups of genes, sequences, structures, or pathways — for batch processing or advanced BLAST use biopython, for multi-database Python workflows use bioservices. Part of the AlterLab Academic Skills suite.

alterlab-ginkgo-cloud

AlterLab-IEU/AlterLab-Academic-Skills

Submits and manages protocols on Ginkgo Bioworks Cloud Lab (cloud.ginkgo.bio), a web-based interface for autonomous lab execution on Reconfigurable Automation Carts (RACs), covering protocol selection, input preparation, pricing, and ordering workflows. Use when running cell-free protein expression (validation or optimization), generating fluorescent pixel art, or interacting with Ginkgo Cloud Lab services. Part of the AlterLab Academic Skills suite.

alterlab-glycoengineering

AlterLab-IEU/AlterLab-Academic-Skills

Analyze and engineer protein glycosylation — scan sequences for N-glycosylation sequons (N-X-S/T), predict O-glycosylation hotspots, and reach curated glycoengineering tools (NetOGlyc, GlycoShield, GlycoWorkbench). Use when identifying or designing glycosylation sites, optimizing therapeutic-antibody or biologic glycoforms, or doing glycoprotein engineering and vaccine-design work. Part of the AlterLab Academic Skills suite.

alterlab-gnomad

AlterLab-IEU/AlterLab-Academic-Skills

Query gnomAD (Genome Aggregation Database) for population allele frequencies and gene constraint scores (pLI, LOEUF) reflecting loss-of-function intolerance. Use when checking how common a variant is across populations, filtering rare-disease candidate variants, assessing variant pathogenicity, or identifying loss-of-function intolerant genes. Part of the AlterLab Academic Skills suite.

alterlab-grant-reporting

AlterLab-IEU/AlterLab-Academic-Skills

Drafts post-award grant deliverables across funder formats — NIH RPPR (Annual/Interim/Final via eRA Commons), NSF annual/final project reports and the public Project Outcomes Report (Research.gov), and Horizon Europe / ERC periodic and final reports (technical Part A/B + financial statements on the EU Funding & Tenders Portal) — plus milestone and deliverable tracking, budget-vs-actual variance narratives, no-cost-extension and rebudgeting justifications, and effort/closeout reporting. Computes report due dates from the award period with scripts/report_deadlines.py. Use when the user needs a grant progress or final report, post-award reporting, an RPPR, a periodic report, a no-cost-extension request, milestone tracking, or a budget-variance narrative. For writing new proposals prefer alterlab-research-grants; for TÜBİTAK proposals prefer alterlab-tubitak-proposal. Part of the AlterLab Academic Skills suite.

alterlab-gtars

AlterLab-IEU/AlterLab-Academic-Skills

Runs high-performance genomic interval analysis with gtars (databio), a Rust toolkit with Python bindings — the performance-critical backend for the geniml ML library. Use when computing overlaps/jaccard/coverage between BED region sets, indexing intervals with IGD, generating uniwig accumulation/coverage tracks, tokenizing genomic regions for ML, splitting single-cell fragments into pseudobulks, or computing GA4GH refget sequence digests. NOT for training region embeddings (use alterlab-geniml) or non-genomic spatial joins (use alterlab-geopandas). Part of the AlterLab Academic Skills suite.

alterlab-gtex

AlterLab-IEU/AlterLab-Academic-Skills

Query the GTEx (Genotype-Tissue Expression) portal v2 REST API for tissue-specific gene expression (median TPM across 54 human tissues), expression QTLs (eQTLs), and splicing QTLs (sQTLs). Use when checking which tissues express a gene, finding which gene a non-coding/GWAS variant regulates via eQTLs, or interpreting variant regulatory effects across tissues. NOT for curated trait-variant associations (use alterlab-gwas), population allele frequencies or variant constraint (use alterlab-gnomad), or gene/transcript structure and ID mapping (use alterlab-ensembl). Part of the AlterLab Academic Skills suite.

alterlab-gwas

AlterLab-IEU/AlterLab-Academic-Skills

Query the NHGRI-EBI GWAS Catalog REST API for SNP-trait associations, retrieving variants by rs ID, disease/trait, or gene along with p-values and summary statistics. Use when investigating genome-wide association study hits, mapping a SNP or rsID to traits, building polygenic risk scores, or doing genetic epidemiology lookups. Part of the AlterLab Academic Skills suite.

alterlab-hedgefund-monitor

AlterLab-IEU/AlterLab-Academic-Skills

Queries the OFR (Office of Financial Research) Hedge Fund Monitor API for time series on hedge fund size, leverage, counterparties, liquidity, complexity, and risk management, including SEC Form PF aggregated statistics, CFTC Traders in Financial Futures, FICC Sponsored Repo volumes, and FRB SCOOS dealer financing terms (no API key or registration required). Use when working with hedge fund data, systemic risk monitoring, financial stability research, hedge fund leverage or leverage ratios, counterparty concentration, Form PF statistics, repo market data, or OFR financial research data. Part of the AlterLab Academic Skills suite.

alterlab-histolab

AlterLab-IEU/AlterLab-Academic-Skills

Extract and preprocess tiles from whole-slide images (WSI) with histolab — OpenSlide-backed slide loading, tissue detection and masks, Random/Grid/Score tile extraction, and image/morphological filters for H&E preprocessing. Use when the user needs lightweight WSI slide preprocessing — building tile datasets for ML training, tissue segmentation, or quick tile-based inspection of histopathology slides. For end-to-end computational-pathology, deep-learning model training, nucleus segmentation, or multiplexed/spatial-proteomics (CODEX, Vectra) pipelines prefer alterlab-pathml instead. Part of the AlterLab Academic Skills suite.

alterlab-hmdb

AlterLab-IEU/AlterLab-Academic-Skills

Access the Human Metabolome Database (HMDB, 220K+ metabolites), searching by name, HMDB ID, or structure to retrieve chemical properties, biomarker data, NMR/MS reference spectra, and associated pathways. Use when identifying a human metabolite, looking up its biomarker or disease associations, matching NMR/MS spectra, or running metabolomics annotation. Part of the AlterLab Academic Skills suite.

alterlab-hypogenic

AlterLab-IEU/AlterLab-Academic-Skills

Runs automated LLM-driven hypothesis generation and testing on tabular datasets with HypoGeniC, combining literature insights with data-driven testing. Use when systematically exploring hypotheses about patterns in empirical data (for example deception detection or content analysis). For manual hypothesis formulation use alterlab-hypothesis-gen; for open-ended creative ideation use alterlab-scientific-brainstorm. Part of the AlterLab Academic Skills suite.

alterlab-hypothesis-gen

AlterLab-IEU/AlterLab-Academic-Skills

Formulates structured, testable hypotheses from experimental observations using a scientific-method framework — derives predictions, proposes mechanisms, and designs experiments to test them, then renders a LaTeX report. Use when turning observations or data into falsifiable, mechanistic hypotheses, competing-explanation sets, testable predictions, or experimental designs. For a standalone systematic literature review or evidence synthesis (not a means to hypotheses) use alterlab-literature-review; to evaluate an existing manuscript use alterlab-peer-review. Part of the AlterLab Academic Skills suite.

alterlab-imaging-data-commons

AlterLab-IEU/AlterLab-Academic-Skills

Query and download public cancer imaging data from the NCI Imaging Data Commons (IDC) using the idc-index Python package, filtering by metadata, visualizing in-browser, and checking licenses, with no authentication required. Use when obtaining large-scale radiology (CT, MR, PET) or digital pathology DICOM datasets for AI/ML training or cancer imaging research. Part of the AlterLab Academic Skills suite.

alterlab-infographics

AlterLab-IEU/AlterLab-Academic-Skills

Creates professional infographics with Nano Banana Pro AI and smart iterative refinement, using Gemini 3 Pro for automated quality review and an optional Perplexity Sonar research phase for accurate, sourced data — supports 10 infographic types, 8 industry styles, and colorblind-safe palettes. Use when the request is for an infographic, data-story graphic, statistical poster, comparison chart, timeline, process/how-to visual, or list/social graphic that pairs a designed layout with figures. Use alterlab-scientific-schematics instead for technical flowcharts, CONSORT/PRISMA, pathways, or architecture diagrams; alterlab-generate-image for non-infographic illustrations. Part of the AlterLab Academic Skills suite.

alterlab-interpro

AlterLab-IEU/AlterLab-Academic-Skills

Query the EMBL-EBI InterPro REST API for protein family, domain, and functional-site annotations integrated from member databases (Pfam, PANTHER, PRINTS, SMART, SUPERFAMILY, CDD, ProSite, NCBIfam, and others). Use when predicting protein function, analyzing or comparing domain architecture, classifying a protein by family or homologous superfamily, resolving a Pfam/InterPro accession, or mapping a protein's signatures to GO terms. Not for raw UniProt entry/FASTA retrieval or AlphaFold 3D structures. Part of the AlterLab Academic Skills suite.

alterlab-iso13485

AlterLab-IEU/AlterLab-Academic-Skills

Prepares ISO 13485 certification documentation for medical device Quality Management Systems (QMS) — gap analysis of existing documentation, Quality Manuals, required procedures and work instructions, and Medical Device Files. Use for ISO 13485 QMS documentation, conducting a documentation gap analysis, drafting a Quality Manual or SOP/work instruction, assembling a Medical Device File, identifying missing documentation for medical device certification, or when medical device regulations, QMS certification, FDA QMSR, or EU MDR are mentioned. Part of the AlterLab Academic Skills suite.

alterlab-jaspar

AlterLab-IEU/AlterLab-Academic-Skills

Query JASPAR for transcription factor binding site (TFBS) profiles (PWMs/PFMs), searching by TF name, species, or class, scanning DNA sequences for binding sites, and comparing matrices. Use when doing motif analysis, regulatory genomics, transcription factor binding prediction, or interpreting regulatory/non-coding GWAS variants. Part of the AlterLab Academic Skills suite.

alterlab-kegg

AlterLab-IEU/AlterLab-Academic-Skills

Provide direct REST API access to KEGG (academic use only) for pathway analysis, gene-to-pathway and compound-to-pathway mapping, metabolic reactions, KEGG Orthology (KO), drug-drug interactions, and ID conversion. Use when querying KEGG pathways, mapping genes/compounds to metabolic maps, or running KEGG pathway enrichment via raw HTTP/REST; for protein-protein interaction networks prefer alterlab-string-db, for protein sequences and annotations prefer alterlab-uniprot, and for Python workflows spanning many databases prefer bioservices instead. Part of the AlterLab Academic Skills suite.

alterlab-kvkk-dmp

AlterLab-IEU/AlterLab-Academic-Skills

Produces KVKK-compliant (Law 6698, as amended by Law 7499 — published in the Official Gazette 12 Mar 2024, KVKK provisions effective 1 Jun 2024) data management plans for Turkish research, encoding the açık rıza (explicit consent) default basis, the Art. 28(1)(b) anonymization exemption as the primary compliance lever, the Art. 6 special-category regime for health/genetic/biometric data, Art. 7 deletion/destruction/anonymization at purpose-end, the Art. 13 thirty-day data-subject response window, Art. 9 cross-border adequacy-decision rules for cloud/overseas data, and Art. 16 VERBIS pre-processing registration, with a KVKK-vs-GDPR crosswalk. Use when the user needs a KVKK data management plan, to anonymize a research dataset under Turkish law, a VERBIS check, or to fix EU DMP boilerplate for Turkey; for pure GDPR/HIPAA use alterlab-research-ethics. Part of the AlterLab Academic Skills suite.

alterlab-labarchive

AlterLab-IEU/AlterLab-Academic-Skills

Integrates the LabArchives electronic lab notebook (ELN) via its REST API — access notebooks, manage entries and attachments, back up notebooks, and bridge to Protocols.io, Jupyter, and REDCap. Use when automating LabArchives ELN workflows, programmatically reading/writing notebook entries or attachments, backing up a LabArchives notebook, or syncing it with Protocols.io, Jupyter, or REDCap. Part of the AlterLab Academic Skills suite.

alterlab-lamindb

AlterLab-IEU/AlterLab-Academic-Skills

Manage, annotate, and trace biological data with LaminDB, an open-source FAIR data framework that makes datasets queryable, versioned, and reproducible. Use when registering or querying biological datasets (scRNA-seq, spatial, flow cytometry), validating and curating data against ontologies (genes, cell types, diseases, tissues), tracking data lineage and computational workflows, building data lakehouses, or wiring integrations with Nextflow, Snakemake, W&B, or MLflow. Part of the AlterLab Academic Skills suite.

alterlab-latchbio

AlterLab-IEU/AlterLab-Academic-Skills

Builds and deploys bioinformatics pipelines on the LatchBio platform using the Latch SDK — author workflows with @workflow/@task decorators, handle LatchFile/LatchDir I/O, register serverless workflows, configure CPU/GPU task resources, organize data in the Latch Registry, and wrap Nextflow/Snakemake pipelines. Use when developing or deploying a Latch SDK workflow, sizing task resources, working with the Registry, or porting a Nextflow/Snakemake bioinformatics pipeline onto LatchBio. Not for DNAnexus (dxpy/dx CLI) or generic Flyte. Part of the AlterLab Academic Skills suite.

alterlab-latex-posters

AlterLab-IEU/AlterLab-Academic-Skills

Creates professional research posters in LaTeX using beamerposter, tikzposter, or baposter — handles layout design, color schemes, multi-column formats, figure integration, and poster-specific visual-communication best practices. Use when building a conference or academic poster in LaTeX. For PowerPoint/PPTX poster output prefer pptx-posters instead. Part of the AlterLab Academic Skills suite.

alterlab-ligandmpnn

AlterLab-IEU/AlterLab-Academic-Skills

Design protein sequences around bound ligands, metals, and nucleic acids with LigandMPNN (Dauparas 2023) — inverse folding that conditions on non-protein context, so binding-pocket and metal-site residues are chosen to fit the actual ligand. Use when designing a small-molecule or metal binding pocket, redesigning residues that contact a ligand/ion/nucleic acid, or doing enzyme active-site design where the substrate matters. For backbone sequence design with NO ligand/metal context prefer alterlab-proteinmpnn; to GENERATE a backbone or scaffold a functional site prefer alterlab-rfdiffusion; to validate a design by refolding prefer alterlab-alphafold; to co-fold or dock the ligand prefer alterlab-boltz or alterlab-diffdock. Part of the AlterLab Academic Skills suite.

alterlab-link-health

AlterLab-IEU/AlterLab-Academic-Skills

Audits and repairs Markdown link health across a skills repo via a four-tier pipeline (config hardening, intra-repo file-ref fixes, external URL substitutions, residual exclusions) and enforces a Tier 3 substitution guardrail that prevents regressions of previously-passing links; designed for lychee-based GitHub Actions link checkers but generalizes to markdown-link-check and similar tools. Use when the request mentions link audit, dead links, link health, lychee, broken links, link checker, markdown link audit, link-health audit, 404 audit, check-links failing, CI link-check, or 連結健檢, 死鏈, 失效連結, 斷鏈檢查. Part of the AlterLab Academic Skills suite.

alterlab-literature-review

AlterLab-IEU/AlterLab-Academic-Skills

Conducts comprehensive, systematic literature reviews across multiple academic databases (PubMed, arXiv, bioRxiv, Semantic Scholar), with PRISMA flow tracking, study screening (title/abstract and full-text), evidence-table extraction, and risk-of-bias assessment, producing professionally formatted markdown documents and PDFs with verified citations in multiple styles (APA, Nature, Vancouver). Use when running a systematic literature review, meta-analysis, research synthesis, or broad literature search, building a PRISMA flow diagram, screening studies, extracting an evidence table, or assessing risk-of-bias across biomedical, scientific, and technical domains. Part of the AlterLab Academic Skills suite.

alterlab-market-research

AlterLab-IEU/AlterLab-Academic-Skills

Generates comprehensive market research reports (50+ pages) in the style of top consulting firms (McKinsey, BCG, Gartner), with professional LaTeX formatting, extensive visuals via scientific-schematics and generate-image, data gathering through research-lookup, and multi-framework strategic analysis (Porter Five Forces, PESTLE, SWOT, TAM/SAM/SOM, BCG Matrix). Use when producing a market analysis, competitive landscape, industry report, market-sizing study, or consulting-style strategic deliverable. NOT for a single focused, source-cited research question with no report/frameworks (use alterlab-deep-research) or for pulling raw financial data points from an API (use alterlab-alpha-vantage). Part of the AlterLab Academic Skills suite.

alterlab-markitdown

AlterLab-IEU/AlterLab-Academic-Skills

Convert files and Office documents to clean, LLM-friendly Markdown with Microsoft MarkItDown (markitdown CLI/Python), supporting PDF, DOCX, PPTX, XLSX, images (EXIF + OCR), audio (transcription), HTML, CSV, JSON, XML, ZIP archives, EPUB e-books, and YouTube transcript URLs, with optional AI image descriptions. Use when converting a document, PDF, slide deck, spreadsheet, scanned image, audio file, web page, or e-book into Markdown text for ingestion or LLM processing, extracting text via OCR, transcribing audio, or batch-converting mixed file formats to token-efficient Markdown. Part of the AlterLab Academic Skills suite.

alterlab-matchms

AlterLab-IEU/AlterLab-Academic-Skills

Computes mass-spectral similarity and identifies compounds for metabolomics with matchms — comparing mass spectra, scoring similarity (cosine, modified cosine), and searching spectral libraries to annotate unknowns. Use when matching MS/MS spectra, identifying metabolites, or library searching; for full LC-MS/MS proteomics pipelines use pyopenms. Part of the AlterLab Academic Skills suite.

alterlab-matplotlib

AlterLab-IEU/AlterLab-Academic-Skills

Builds plots with the matplotlib Python library (pyplot and the object-oriented Figure/Axes API) for full low-level customization, exporting to PNG/PDF/SVG. Use when fine-grained control over individual plot elements is needed — custom line/scatter/bar/histogram/heatmap/contour/box/violin/3D plots, rcParams and style-sheet tuning, or GridSpec subplot layouts inside a scientific Python workflow. Does NOT cover opinionated journal-ready multi-panel figure workflows (Nature/Science/Cell formatting, colorblind-safe palettes, significance annotations); for those prefer alterlab-scientific-viz instead. Part of the AlterLab Academic Skills suite.

alterlab-medchem

AlterLab-IEU/AlterLab-Academic-Skills

Applies medicinal-chemistry filters with the medchem library — drug-likeness rules (Lipinski, Veber), PAINS filters, structural alerts, and molecular complexity metrics for compound prioritization and library cleanup. Use when filtering or triaging a compound library, flagging PAINS or reactive groups, or assessing drug-likeness of candidate molecules. Part of the AlterLab Academic Skills suite.

alterlab-mermaid

AlterLab-IEU/AlterLab-Academic-Skills

Writes Markdown documents and text-based Mermaid diagrams (flowcharts, sequence, class, ER, gantt, state, and more) with full style guides, 24 diagram-type references, and 9 document templates. Use when authoring a scientific document, report, analysis, or README, or when a diagram should be expressed as version-controllable Mermaid/Markdown text rather than a rendered image. For AI-rendered publication schematics use scientific-schematics instead. Part of the AlterLab Academic Skills suite.

alterlab-meta-analysis

AlterLab-IEU/AlterLab-Academic-Skills

Runs quantitative meta-analysis — computes effect sizes (Hedges' g / standardized mean difference, log odds/risk ratios) with their variances, pools them under fixed-effect and random-effects models, quantifies heterogeneity (I-squared, tau-squared, Cochran's Q), draws forest and funnel plots, and tests publication bias (Egger's regression, trim-and-fill) — using statsmodels.stats.meta_analysis in Python or the field-standard R metafor via Rscript. It enforces PRISMA reporting and the random- vs fixed-effect decision. Use when pooling effect sizes across studies, running a systematic review's quantitative synthesis, or assessing heterogeneity and publication bias. For finding and screening the literature prefer alterlab-deep-research; for a single study's statistics prefer alterlab-statistical-analysis. Part of the AlterLab Academic Skills suite.

alterlab-metabolomics-wb

AlterLab-IEU/AlterLab-Academic-Skills

Access the NIH Metabolomics Workbench via its REST API (4,200+ studies), querying metabolites, RefMet standardized nomenclature, MS/NMR data, m/z mass searches, and study metadata. Use when retrieving public metabolomics study data, standardizing metabolite names with RefMet, running m/z lookups, or doing biomarker discovery. Part of the AlterLab Academic Skills suite.

alterlab-missing-data

AlterLab-IEU/AlterLab-Academic-Skills

Handles missing data with principled methods — forces an explicit MCAR / MAR / MNAR mechanism statement, then applies multiple imputation by chained equations (MICE) with Rubin's-rules pooling of estimates and standard errors, or full-information maximum likelihood (FIML) where a likelihood/SEM model applies. Uses statsmodels MICE / MICEData in Python or the field-standard R mice via Rscript, and warns that single (mean/regression) imputation and scikit-learn's IterativeImputer return one completed dataset without Rubin's-rules pooling, so they understate standard errors if used as multiple imputation. Use when a dataset has missing values, when choosing an imputation strategy, or when reporting how missingness was handled. For general modeling on complete data prefer alterlab-statistical-analysis; for latent-variable models with FIML prefer alterlab-sem-psychometrics. Part of the AlterLab Academic Skills suite.

alterlab-mixed-methods

AlterLab-IEU/AlterLab-Academic-Skills

Mixed methods research design and integration strategies for combining qualitative and quantitative approaches. Use when planning convergent, explanatory sequential, exploratory sequential, embedded, transformative, or multiphase designs; when integrating diverse data sources through merging, connecting, or embedding; when constructing joint displays or meta-inferences; or when evaluating quality criteria specific to mixed methods research. Covers Creswell & Plano Clark frameworks, notation systems, and software tools for integration. For single-strand qualitative coding (thematic analysis, grounded theory, saturation, inter-coder reliability) use alterlab-qualitative-methods; for questionnaire/Likert/instrument-validation mechanics use alterlab-survey-design. Part of the AlterLab Academic Skills suite.

alterlab-modal

AlterLab-IEU/AlterLab-Academic-Skills

Runs Python code in the cloud with Modal — serverless containers, on-demand GPUs, and autoscaling. Use when deploying ML models, running batch processing jobs, scheduling compute-intensive tasks, or serving APIs that need GPU acceleration or dynamic scaling. Part of the AlterLab Academic Skills suite.

alterlab-molecular-dynamics

AlterLab-IEU/AlterLab-Academic-Skills

Runs and analyzes molecular dynamics simulations with OpenMM and MDAnalysis — setting up protein and small-molecule systems, assigning force fields, running energy minimization and production MD, and analyzing trajectories (RMSD, RMSF, contact maps, free energy surfaces). Use when simulating protein or ligand dynamics, equilibrating a system, or computing trajectory metrics for structural biology, drug binding, or biophysics. Part of the AlterLab Academic Skills suite.

alterlab-molfeat

AlterLab-IEU/AlterLab-Academic-Skills

Featurizes molecules for machine learning with molfeat (100+ featurizers) — ECFP/MACCS/MAP4 fingerprints, RDKit and Mordred physicochemical descriptors, and pretrained embeddings (ChemBERTa, ChemGPT, GIN) exposed as scikit-learn transformers that convert SMILES into feature vectors. Use when turning molecules into ML-ready feature matrices for QSAR/QSPR or virtual screening, or benchmarking fingerprint against descriptor and embedding representations; for training models and MoleculeNet benchmarks on those features prefer alterlab-deepchem, and for low-level fingerprint or descriptor primitives prefer alterlab-rdkit. Part of the AlterLab Academic Skills suite.

alterlab-monarch

AlterLab-IEU/AlterLab-Academic-Skills

Query the Monarch Initiative knowledge graph for disease-gene-phenotype associations across species, integrating OMIM, ORPHANET, HPO, ClinVar, and model organism databases. Use when discovering rare disease genes, mapping phenotypes to genes, modeling disease across species, or looking up HPO terms. Part of the AlterLab Academic Skills suite.

alterlab-multilevel-models

AlterLab-IEU/AlterLab-Academic-Skills

Fits and reports mixed-effects / multilevel / hierarchical models for clustered, nested, longitudinal, and repeated-measures data — random intercepts and slopes, variance components and the ICC, cross-level interactions, and GLMMs (logistic/Poisson) — using statsmodels MixedLM and bambi (Bayesian on PyMC) in Python, or the field-standard R lme4 / glmmTMB / brms via Rscript. It enforces the reporting items reviews find under-reported: full fixed + random specification, centering, variance components + ICC, estimation method, assumption checks, model comparisons, and effect sizes. Use when data are grouped/nested (students in schools, repeated measures, panel/longitudinal) and the question concerns within- vs between-cluster variation. For general single-level regression prefer alterlab-statsmodels; for panel fixed effects used for causal identification prefer alterlab-causal-inference. Part of the AlterLab Academic Skills suite.

alterlab-networkx

AlterLab-IEU/AlterLab-Academic-Skills

Creates, analyzes, and visualizes complex networks and graphs in Python with NetworkX. Use when working with network/graph data structures, analyzing relationships between entities, computing graph algorithms (shortest paths, centrality, clustering), detecting communities, generating synthetic networks, or visualizing topologies — applicable to social, biological, transportation, citation, and any pairwise-relationship networks. This is classical graph analytics, not deep learning — for training graph neural networks (GCN/message passing, node/edge/graph classification on Cora-style data) use alterlab-torch-geometric instead. Part of the AlterLab Academic Skills suite.

alterlab-neurokit2

AlterLab-IEU/AlterLab-Academic-Skills

Processes and analyzes physiological biosignals with the NeuroKit2 Python toolkit — ECG, EEG, EDA, RSP, PPG, EMG, and EOG signals. Use when processing cardiovascular signals, brain activity, electrodermal responses, respiratory patterns, muscle activity, or eye movements, or when computing heart rate variability (HRV), event-related potentials, complexity measures, autonomic nervous system assessment, or multi-modal physiological signal integration for psychophysiology research. Part of the AlterLab Academic Skills suite.

alterlab-neuropixels

AlterLab-IEU/AlterLab-Academic-Skills

Analyze Neuropixels 1.0/2.0 extracellular electrophysiology with SpikeInterface — load SpikeGLX/Open Ephys recordings, preprocess and motion-correct, run Kilosort4 spike sorting, compute quality metrics, apply Allen/IBL curation, and do AI-assisted visual inspection. Use when working with neural recordings, spike sorting, or extracellular electrophysiology, or when the user mentions Neuropixels, SpikeGLX, Open Ephys, Kilosort, quality metrics, or unit curation. Part of the AlterLab Academic Skills suite.

alterlab-nf-core-sarek

AlterLab-IEU/AlterLab-Academic-Skills

Runs FASTQ-to-VCF germline and somatic variant calling via the Nextflow nf-core/sarek pipeline pinned to -r 3.8.1 — builds the samplesheet.csv (patient, sex, status, sample, lane, fastq_1, fastq_2), runs bwa-mem/bwa-mem2/dragmap alignment plus GATK4 MarkDuplicates and BQSR against the GATK GRCh38 resource bundle (dbSNP, Mills/1000G indels), and selects callers — explicitly correcting that sarek defaults to Strelka when --tools is unset (pass haplotypecaller for GATK best practice or deepvariant for CNN accuracy), with a non-Nextflow manual GATK4 fallback. Use when the user wants a variant-calling pipeline, FASTQ to VCF, germline or somatic SNV/indel calling, nf-core/sarek, GATK best-practices alignment-to-VCF, or BQSR/HaplotypeCaller/Mutect2/DeepVariant; annotate hits with alterlab-clinvar/alterlab-gnomad/alterlab-cosmic, parse VCFs with alterlab-pysam, store at scale with alterlab-tiledbvcf. Part of the AlterLab Academic Skills suite.

alterlab-omero

AlterLab-IEU/AlterLab-Academic-Skills

Manages microscopy image data on an OMERO server via the OMERO Python API (BlitzGateway) — access images, retrieve datasets, read pixel data, manage ROIs and annotations, and batch-process. Use when connecting to an OMERO server, pulling microscopy images or datasets, analyzing pixels, managing ROIs/annotations, or running high-content screening and microscopy workflows. Part of the AlterLab Academic Skills suite.

alterlab-open-notebook

AlterLab-IEU/AlterLab-Academic-Skills

Run Open Notebook, a self-hosted open-source alternative to Google NotebookLM with a full REST API, for AI-powered research and document analysis. Use when organizing research materials into notebooks, ingesting diverse content sources (PDFs, videos, audio, web pages, Office documents), generating AI-powered notes and summaries, creating multi-speaker podcasts from research, chatting with documents using context-aware AI, searching across materials with full-text and vector search, or running custom content transformations. Supports 18+ AI providers including OpenAI, Anthropic, Google, Ollama, LM Studio, Groq, and Mistral with complete data privacy through self-hosting. For a one-shot file-to-Markdown conversion (no notebook, chat, or search), use alterlab-markitdown instead. Part of the AlterLab Academic Skills suite.

alterlab-open-science

AlterLab-IEU/AlterLab-Academic-Skills

Guidance for open science practices — preregistration (OSF Registries, AsPredicted, PROSPERO, ClinicalTrials.gov), open data and FAIR principles, repository choice (Zenodo, Dryad, Figshare), open access routes (Green/Gold/Diamond), Creative Commons licensing, reproducible computational workflows (Docker, Binder, Code Ocean), registered reports, open peer review, and TOP Guidelines. Use when preregistering a study, writing the FAIR data-sharing and repository section of a grant data management plan (NSF, NIH, ERC, UKRI), choosing a data repository, navigating open access, or building reproducible analyses. For human-subjects ethics, IRB applications, informed consent, or GDPR/HIPAA compliance, defer to alterlab-research-ethics. Part of the AlterLab Academic Skills suite.