Browse Skills — Page 43

bio-clinical-biostatistics-logistic-regression

GPTomics/bioSkills

Performs logistic regression for clinical trial outcomes including binary, ordinal, and multinomial models. Extracts odds ratios with confidence intervals, handles covariate adjustment, and provides Firth penalized regression for rare events or separation. Use when modeling binary or ordinal endpoints from clinical data.

bio-clinical-biostatistics-subgroup-analysis

GPTomics/bioSkills

Performs stratified and subgroup analyses for clinical trial data. Covers Mantel-Haenszel pooling, Breslow-Day homogeneity testing, interaction terms in regression, multiple comparisons correction, and forest plot visualization. Use when analyzing treatment effects across patient subgroups or controlling for stratification variables.

bio-clinical-biostatistics-trial-reporting

GPTomics/bioSkills

Prepares statistical tables and reports for clinical trials following regulatory standards. Generates Table 1 baseline characteristics, defines analysis populations (ITT, per-protocol, safety), performs multiple imputation for missing data, and follows CONSORT and ICH E9 guidelines. Use when creating analysis reports, handling missing data, or preparing regulatory submissions from clinical trials.

bio-clinical-databases-clinvar-lookup

GPTomics/bioSkills

Query ClinVar for variant pathogenicity classifications, review status, and disease associations via REST API or local VCF. Use when determining clinical significance of variants for diagnostic or research purposes.

bio-clinical-databases-dbsnp-queries

GPTomics/bioSkills

Query dbSNP for rsID lookups, variant annotations, and cross-references to other databases. Use when mapping between rsIDs and genomic coordinates or retrieving basic variant information.

bio-clinical-databases-gnomad-frequencies

GPTomics/bioSkills

Query gnomAD for population allele frequencies to assess variant rarity. Use when filtering variants by population frequency for rare disease analysis or determining if a variant is common in the general population.

bio-clinical-databases-hla-typing

GPTomics/bioSkills

Call HLA alleles from NGS data using OptiType, HLA-HD, or arcasHLA for immunogenomics applications. Use when determining HLA genotype for transplant matching, neoantigen prediction, or pharmacogenomic screening.

bio-clinical-databases-myvariant-queries

GPTomics/bioSkills

Query myvariant.info API for aggregated variant annotations from multiple databases (ClinVar, gnomAD, dbSNP, COSMIC, etc.) in a single request. Use when annotating variants with clinical and population data from multiple sources simultaneously.

bio-clinical-databases-pharmacogenomics

GPTomics/bioSkills

Query PharmGKB and CPIC for drug-gene interactions, pharmacogenomic annotations, and dosing guidelines. Use when predicting drug response from genetic variants or implementing clinical pharmacogenomics.

bio-clinical-databases-polygenic-risk

GPTomics/bioSkills

Calculate polygenic risk scores using PRSice-2, LDpred2, or PRS-CS from GWAS summary statistics. Use when predicting disease risk from genome-wide genetic variants.

bio-clinical-databases-somatic-signatures

GPTomics/bioSkills

Extract and analyze mutational signatures from somatic variants using SigProfiler or MutationalPatterns to characterize mutagenic processes. Use when identifying DNA damage mechanisms or etiology in cancer genomes.

bio-clinical-databases-tumor-mutational-burden

GPTomics/bioSkills

Calculate tumor mutational burden from panel or WES data with proper normalization and clinical thresholds. Use when assessing immunotherapy eligibility or characterizing tumor immunogenicity.

bio-clinical-databases-variant-prioritization

GPTomics/bioSkills

Filter and prioritize variants by pathogenicity, population frequency, and clinical evidence for rare disease analysis. Use when identifying candidate disease-causing variants from exome or genome sequencing.

bio-clip-seq-binding-site-annotation

GPTomics/bioSkills

Annotate CLIP-seq binding sites to genomic features including 3'UTR, 5'UTR, CDS, introns, and ncRNAs. Use when characterizing where an RBP binds in transcripts.

bio-clip-seq-clip-alignment

GPTomics/bioSkills

Align CLIP-seq reads to the genome with crosslink site awareness. Use when mapping preprocessed CLIP reads for peak calling.

bio-clip-seq-clip-motif-analysis

GPTomics/bioSkills

Identify enriched sequence motifs at CLIP-seq binding sites for RBP binding specificity. Use when characterizing the sequence preferences of an RNA-binding protein.

bio-clip-seq-clip-peak-calling

GPTomics/bioSkills

Call protein-RNA binding site peaks from CLIP-seq data using CLIPper, PureCLIP, or Piranha. Use when identifying RBP binding sites from aligned CLIP reads.

bio-clip-seq-clip-preprocessing

GPTomics/bioSkills

Preprocess CLIP-seq data including adapter trimming, UMI extraction, and PCR duplicate removal. Use when preparing raw CLIP, iCLIP, or eCLIP reads for peak calling.

bio-codon-usage

GPTomics/bioSkills

Analyze codon usage, calculate CAI (Codon Adaptation Index), and examine synonymous codon bias using Biopython. Use when analyzing coding sequences for expression optimization or evolutionary analysis.

bio-comparative-genomics-ancestral-reconstruction

GPTomics/bioSkills

Reconstruct ancestral sequences at phylogenetic nodes using PAML and IQ-TREE marginal likelihood methods. Infer ancient protein sequences and trace evolutionary trajectories through sequence history. Use when inferring ancestral states for protein resurrection or tracing evolutionary history.

bio-comparative-genomics-hgt-detection

GPTomics/bioSkills

Detect horizontal gene transfer events using HGTector, compositional analysis, and phylogenetic incongruence methods. Identify foreign genes in bacterial and archaeal genomes from anomalous composition or unexpected phylogenetic placement. Use when searching for horizontally transferred genes or analyzing genome evolution in prokaryotes.

bio-comparative-genomics-ortholog-inference

GPTomics/bioSkills

Infer orthologous gene groups across species using OrthoFinder and ProteinOrtho. Identify orthologs, paralogs, and co-orthologs for comparative genomics and functional annotation transfer. Use when identifying gene orthologs across species or building orthogroups for evolutionary analysis.

bio-comparative-genomics-positive-selection

GPTomics/bioSkills

Detect positive selection using dN/dS (omega) tests with PAML codeml and HyPhy. Identify sites and branches under adaptive evolution through codon models and branch-site tests. Use when testing for adaptive evolution in gene families or identifying positively selected sites.

bio-comparative-genomics-synteny-analysis

GPTomics/bioSkills

Analyze genome collinearity and syntenic blocks using MCScanX, SyRI, and JCVI for comparative genomics. Detect conserved gene order, chromosomal rearrangements, and whole-genome duplications. Use when comparing genome structure between species or identifying conserved genomic regions.

bio-compressed-files

GPTomics/bioSkills

Read and write compressed sequence files (gzip, bzip2, BGZF) using Biopython. Use when working with .gz or .bz2 sequence files. Use BGZF for indexable compressed files.

bio-consensus-sequences

GPTomics/bioSkills

Generate consensus FASTA sequences by applying VCF variants to a reference using bcftools consensus. Use when creating sample-specific reference sequences or reconstructing haplotypes.

bio-copy-number-cnv-annotation

GPTomics/bioSkills

Annotate CNVs with genes, pathways, and clinical significance. Use when interpreting CNV calls or identifying affected genes from copy number analysis.

bio-copy-number-cnv-visualization

GPTomics/bioSkills

Visualize copy number profiles, segments, and compare across samples. Create publication-quality plots of CNV data from CNVkit, GATK, or other callers. Use when creating genome-wide CNV plots, sample heatmaps, or chromosome-level visualizations.

bio-copy-number-cnvkit-analysis

GPTomics/bioSkills

Detect copy number variants from targeted/exome sequencing using CNVkit. Supports tumor-normal pairs, tumor-only, and germline CNV calling. Use when detecting CNVs from WES or targeted panel sequencing data.

bio-copy-number-gatk-cnv

GPTomics/bioSkills

Call copy number variants using GATK best practices workflow. Supports both somatic (tumor-normal) and germline CNV detection from WGS or WES data. Use when following GATK best practices or integrating CNV calling with other GATK variant pipelines.

bio-crispr-screens-base-editing-analysis

GPTomics/bioSkills

Analyzes base editing and prime editing outcomes including editing efficiency, bystander edits, and indel frequencies. Use when quantifying CRISPR base editor results, comparing ABE vs CBE efficiency, or assessing prime editing fidelity.

bio-crispr-screens-batch-correction

GPTomics/bioSkills

Batch effect correction for CRISPR screens. Covers normalization across batches, technical replicate handling, and batch-aware analysis. Use when combining screens from multiple batches or correcting systematic technical variation.

bio-crispr-screens-crispresso-editing

GPTomics/bioSkills

CRISPResso2 for analyzing CRISPR gene editing outcomes. Quantifies indels, HDR efficiency, and generates comprehensive editing reports. Use when analyzing amplicon sequencing data from CRISPR editing experiments to assess editing efficiency.

bio-crispr-screens-hit-calling

GPTomics/bioSkills

Statistical methods for calling hits in CRISPR screens. Covers MAGeCK, BAGEL2, drugZ, and custom approaches for identifying essential and resistance genes. Use when identifying significant genes from screen count data after QC passes.

bio-crispr-screens-jacks-analysis

GPTomics/bioSkills

JACKS (Joint Analysis of CRISPR/Cas9 Knockout Screens) for modeling sgRNA efficacy and gene essentiality. Use when analyzing multiple CRISPR screens simultaneously or when accounting for variable sgRNA efficiency across experiments.

bio-crispr-screens-library-design

GPTomics/bioSkills

CRISPR library design for genetic screens. Covers sgRNA selection, library composition, control design, and oligo ordering. Use when designing custom sgRNA libraries for knockout, activation, or interference screens.

bio-crispr-screens-mageck-analysis

GPTomics/bioSkills

MAGeCK (Model-based Analysis of Genome-wide CRISPR-Cas9 Knockout) for pooled CRISPR screen analysis. Covers count normalization, gene ranking, and pathway analysis. Use when identifying essential genes, drug targets, or resistance mechanisms from dropout or enrichment screens.

bio-crispr-screens-screen-qc

GPTomics/bioSkills

Quality control for pooled CRISPR screens. Covers library representation, read distribution, replicate correlation, and essential gene recovery. Use when assessing screen quality before hit calling or diagnosing poor screen performance.

bio-ctdna-mutation-detection

GPTomics/bioSkills

Detects somatic mutations in circulating tumor DNA using variant callers optimized for low allele fractions with UMI-based error suppression. Reliably detects mutations at VAF above 0.5 percent using consensus-based approaches. Use when identifying tumor mutations from plasma DNA or tracking specific variants.

bio-data-visualization-circos-plots

GPTomics/bioSkills

Create circular genome visualizations with Circos and pyCircos. Display multi-track data including ideograms, genes, variants, CNVs, and interaction arcs. Use when creating circular genome visualizations.

bio-data-visualization-color-palettes

GPTomics/bioSkills

Select and apply colorblind-friendly palettes for scientific figures using viridis, RColorBrewer, and custom color schemes. Use when selecting colorblind-friendly palettes for figures.

bio-data-visualization-genome-browser-tracks

GPTomics/bioSkills

Generate genome browser visualizations using pyGenomeTracks or IGV batch scripting for publication figures. Use when creating publication figures of genomic regions with multiple data tracks.

bio-data-visualization-genome-tracks

GPTomics/bioSkills

Create genome browser-style visualizations showing multiple data tracks (coverage, peaks, genes) using pyGenomeTracks, Gviz, and IGV. Use when visualizing genomic data at specific loci with multiple aligned tracks.

bio-data-visualization-ggplot2-fundamentals

GPTomics/bioSkills

Create publication-quality scientific figures with ggplot2 including scatter plots, boxplots, heatmaps, and multi-panel layouts. Use when creating static figures for papers, presentations, or reports in R.

bio-data-visualization-heatmaps-clustering

GPTomics/bioSkills

Create clustered heatmaps with row/column annotations using ComplexHeatmap, pheatmap, and seaborn for gene expression and omics data visualization. Use when visualizing expression patterns across samples or identifying co-expressed gene clusters.

bio-data-visualization-interactive-visualization

GPTomics/bioSkills

Create interactive HTML plots with plotly and bokeh for exploratory data analysis and web-based sharing of omics visualizations. Use when building zoomable, hoverable plots for data exploration or web dashboards.

bio-data-visualization-multipanel-figures

GPTomics/bioSkills

Combine multiple plots into publication-ready multi-panel figures using patchwork, cowplot, or matplotlib GridSpec with shared legends and panel labels. Use when combining multiple plots into publication figures.

bio-data-visualization-network-visualization

GPTomics/bioSkills

Visualize biological networks including gene regulatory networks, protein interaction networks, and co-expression modules using NetworkX, PyVis, and Cytoscape automation. Produces interactive and publication-quality network figures. Use when creating network diagrams from interaction data, GRN results, or co-expression modules.

bio-data-visualization-specialized-omics-plots

GPTomics/bioSkills

Reusable plotting functions for common omics visualizations. Custom ggplot2/matplotlib implementations of volcano, MA, PCA, enrichment dotplots, boxplots, and survival curves. Use when creating volcano, MA, or enrichment plots.

bio-data-visualization-upset-plots

GPTomics/bioSkills

Create UpSet plots to visualize set intersections as an alternative to Venn diagrams using UpSetR or upsetplot. Use when comparing overlapping gene sets, peak sets, or sample groups with more than 3 sets.

bio-data-visualization-volcano-customization

GPTomics/bioSkills

Create publication-ready volcano plots with custom thresholds, gene labels, and highlighting using ggplot2, EnhancedVolcano, or matplotlib. Use when visualizing differential expression or association results with gene annotations.

bio-de-deseq2-basics

GPTomics/bioSkills

Perform differential expression analysis using DESeq2 in R/Bioconductor. Use for analyzing RNA-seq count data, creating DESeqDataSet objects, running the DESeq workflow, and extracting results with log fold change shrinkage. Use when performing DE analysis with DESeq2.

bio-de-edger-basics

GPTomics/bioSkills

Perform differential expression analysis using edgeR in R/Bioconductor. Use for analyzing RNA-seq count data with the quasi-likelihood F-test framework, creating DGEList objects, normalization, dispersion estimation, and statistical testing. Use when performing DE analysis with edgeR.

bio-de-results

GPTomics/bioSkills

Extract, filter, annotate, and export differential expression results from DESeq2 or edgeR. Use for identifying significant genes, applying multiple testing corrections, adding gene annotations, and preparing results for downstream analysis. Use when filtering and exporting DE analysis results.

bio-de-visualization

GPTomics/bioSkills

Visualize differential expression results using DESeq2/edgeR built-in functions. Covers plotMA, plotDispEsts, plotCounts, plotBCV, sample distance heatmaps, and p-value histograms. Use when visualizing differential expression results.

bio-differential-expression-timeseries-de

GPTomics/bioSkills

Analyze time-series RNA-seq data using limma voom with splines, maSigPro, and ImpulseDE2. Identify genes with dynamic expression patterns. Use when analyzing time-series or longitudinal expression data.

bio-differential-splicing

GPTomics/bioSkills

Detects differential alternative splicing between conditions using rMATS-turbo (binomial LRT on junction counts), leafcutter (Dirichlet-multinomial GLM on intron clusters), MAJIQ V3 deltapsi/HET (Bayesian posterior on LSVs), SUPPA2 (empirical-null on TPM-derived PSI), or Shiba (junction-imbalance-corrected, 2025 SOTA at low coverage). Reports FDR-corrected significance and delta PSI effect sizes. Tools differ in statistical model, annotation dependence, calibration regime, and replicate-count requirements. Use when comparing splicing patterns between treatment groups, tissues, or disease states.

bio-duplicate-handling

GPTomics/bioSkills

Mark and remove PCR/optical duplicates using samtools fixmate and markdup. Use when preparing alignments for variant calling or when duplicate reads would bias analysis.

bio-ecological-genomics-biodiversity-metrics

GPTomics/bioSkills

Calculates species richness, diversity, and turnover using the Hill number framework with iNEXT coverage-based rarefaction/extrapolation, asymptotic diversity estimation, and beta diversity partitioning (betapart turnover vs nestedness). Compares assemblages using coverage-standardized rather than size-standardized rarefaction. Use when quantifying biodiversity from species abundance or incidence data, comparing diversity across sites, or constructing rarefaction curves. Not for clinical 16S microbiome alpha/beta diversity (see microbiome/diversity-analysis).

bio-ecological-genomics-community-ecology

GPTomics/bioSkills

Analyzes community composition using constrained ordination (CCA, RDA, db-RDA), variance partitioning (varpart), indicator species analysis (indicspecies multipatt), and distance-based environmental gradient methods with vegan. Links species composition to environmental explanatory variables. Use when testing how environmental gradients structure species communities, identifying habitat indicator taxa, or partitioning explained variation among predictors. Not for basic unconstrained ordination and PERMANOVA (see microbiome/diversity-analysis).

bio-ecological-genomics-conservation-genetics

GPTomics/bioSkills

Assesses genetic health of populations for conservation using effective population size estimation (GONE2 for recent Ne trajectory, NeEstimator for contemporary Ne, Stairway Plot 2 and PSMC for historical Ne), F-statistics (hierfstat), runs of homozygosity (detectRUNS), and genetic diversity metrics. Use when estimating effective population size, detecting inbreeding or bottlenecks, or assessing genetic diversity in threatened species from microsatellite or SNP data.

bio-ecological-genomics-edna-metabarcoding

GPTomics/bioSkills

Processes environmental DNA metabarcoding data from raw amplicon reads to species occurrence tables using OBITools3, DADA2, and taxonomic assignment against BOLD, MIDORI2, or MitoFish databases. Handles COI, 12S, rbcL, and ITS barcode regions with primer removal, denoising, chimera detection, and contamination filtering via decontam. Includes occupancy modeling (occumb) for detection probability correction. Use when analyzing eDNA from water, soil, or bulk samples for biodiversity monitoring. Not for 16S human microbiome (see microbiome/amplicon-processing).

bio-ecological-genomics-landscape-genomics

GPTomics/bioSkills

Tests genotype-environment associations and identifies loci under local adaptation using LFMM2 (LEA), pcadapt outlier detection, OutFLANK Fst-based selection scans, and redundancy analysis. Detects adaptive genetic variation correlated with environmental variables while controlling for population structure. Use when identifying adaptive loci across environmental gradients, testing for signatures of local adaptation, or predicting genetic vulnerability to climate change with gradientForest.

bio-ecological-genomics-species-delimitation

GPTomics/bioSkills

Delimits species boundaries from molecular data using distance-based (ASAP), tree-based (bPTP, GMYC), and coalescent (BPP) methods. Compares multiple delimitation results with delimtools. Use when delineating putative species from DNA barcoding data, resolving cryptic species complexes, or validating taxonomic assignments. Emphasizes multi-method consensus following integrative taxonomy best practice.

bio-entrez-fetch

GPTomics/bioSkills

Retrieve records from NCBI databases using Biopython Bio.Entrez. Use when downloading sequences, fetching GenBank records, getting document summaries, or parsing NCBI data into Biopython objects.

bio-entrez-link

GPTomics/bioSkills

Find cross-references between NCBI databases using Biopython Bio.Entrez. Use when navigating from genes to proteins, sequences to publications, finding related records, or discovering database relationships.

bio-entrez-search

GPTomics/bioSkills

Search NCBI databases using Biopython Bio.Entrez. Use when finding records by keyword, building complex search queries, discovering database structure, or getting global query counts across databases.

bio-epidemiological-genomics-amr-surveillance

GPTomics/bioSkills

Detect and track antimicrobial resistance genes using AMRFinderPlus and ResFinder with epidemiological context. Monitor resistance trends and identify emerging resistance patterns. Use when screening genomes for AMR genes or tracking resistance in surveillance programs.

bio-epidemiological-genomics-pathogen-typing

GPTomics/bioSkills

Perform multi-locus sequence typing (MLST), core genome MLST, and SNP-based strain typing for bacterial isolate characterization using mlst and chewBBACA. Use when identifying strain types, tracking outbreak clones, or characterizing bacterial isolates.

bio-epidemiological-genomics-phylodynamics

GPTomics/bioSkills

Construct time-scaled phylogenies and infer evolutionary dynamics using TreeTime and BEAST2 for outbreak analysis. Estimate divergence times, molecular clock rates, and ancestral states. Use when dating outbreak origins, estimating transmission rates, or building time-calibrated trees.

bio-epidemiological-genomics-transmission-inference

GPTomics/bioSkills

Infer pathogen transmission networks and identify likely transmission pairs using TransPhylo and outbreak reconstruction algorithms. Estimate who-infected-whom from genomic and epidemiological data. Use when investigating outbreak transmission chains or identifying superspreaders.

bio-epidemiological-genomics-variant-surveillance

GPTomics/bioSkills

Assign pathogen lineages and track variants using Nextclade and pangolin for viral surveillance. Monitor variant prevalence and identify emerging variants of concern. Use when classifying viral sequences, tracking lineage dynamics, or monitoring for variants of concern.

bio-epitranscriptomics-m6a-differential

GPTomics/bioSkills

Identify differential m6A methylation between conditions from MeRIP-seq. Use when comparing epitranscriptomic changes between treatment groups or cell states.

bio-epitranscriptomics-m6a-peak-calling

GPTomics/bioSkills

Call m6A peaks from MeRIP-seq IP vs input comparisons. Use when identifying m6A modification sites from methylated RNA immunoprecipitation data.

bio-epitranscriptomics-m6anet-analysis

GPTomics/bioSkills

Detect m6A modifications from Oxford Nanopore direct RNA sequencing using m6Anet. Use when analyzing epitranscriptomic modifications from long-read RNA data without immunoprecipitation.

bio-epitranscriptomics-merip-preprocessing

GPTomics/bioSkills

Align and QC MeRIP-seq IP and input samples for m6A analysis. Use when preparing MeRIP-seq data for peak calling or differential methylation analysis.

bio-epitranscriptomics-modification-visualization

GPTomics/bioSkills

Create metagene plots and browser tracks for RNA modification data. Use when visualizing m6A distribution patterns around genomic features like stop codons.

bio-experimental-design-batch-design

GPTomics/bioSkills

Designs experiments to minimize and account for batch effects using balanced layouts and blocking strategies. Use when planning multi-batch experiments, assigning samples to sequencing lanes, or designing studies where technical variation could confound biological signals.

bio-experimental-design-multiple-testing

GPTomics/bioSkills

Applies multiple testing correction methods including FDR, Bonferroni, and q-value for genomics data. Use when filtering differential expression results, setting significance thresholds, or choosing between correction methods for different study designs.

bio-experimental-design-power-analysis

GPTomics/bioSkills

Calculates statistical power and minimum sample sizes for RNA-seq, ATAC-seq, and other sequencing experiments. Use when planning experiments, determining how many replicates are needed, or assessing whether a study is adequately powered to detect expected effect sizes.

bio-experimental-design-sample-size

GPTomics/bioSkills

Estimates required sample sizes for differential expression, ChIP-seq, methylation, and proteomics studies. Use when budgeting experiments, writing grant proposals, or determining minimum replicates needed to achieve statistical significance for expected effect sizes.

bio-expression-matrix-counts-ingest

GPTomics/bioSkills

Load gene expression count matrices from various formats including CSV, TSV, featureCounts, Salmon, kallisto, and 10X. Use when importing quantification results for downstream analysis.

bio-expression-matrix-gene-id-mapping

GPTomics/bioSkills

Convert between gene identifier systems including Ensembl, Entrez, HGNC symbols, and UniProt. Use when mapping IDs for pathway analysis or matching different data sources.

bio-expression-matrix-metadata-joins

GPTomics/bioSkills

Merge sample metadata with count matrices and add gene annotations. Use when preparing data for differential expression analysis or visualization.

bio-expression-matrix-normalization

GPTomics/bioSkills

Normalize and transform RNA-seq count matrices for differential expression, visualization, and clustering. Covers between-sample (TMM, RLE, upper quartile), within-sample (TPM, FPKM), variance-stabilizing (VST, rlog), and single-cell (scran) methods. Use when choosing or applying normalization to expression data.

bio-expression-matrix-sparse-handling

GPTomics/bioSkills

Work with sparse matrices for memory-efficient storage of count data. Use when dealing with single-cell data or large bulk RNA-seq datasets where most values are zero.

bio-fastq-quality

GPTomics/bioSkills

Work with FASTQ quality scores using Biopython. Use when analyzing read quality, filtering by quality, trimming low-quality bases, or generating quality reports.

bio-filter-sequences

GPTomics/bioSkills

Filter and select sequences by criteria (length, ID, GC content, patterns) using Biopython. Use when subsetting sequences, removing unwanted records, or selecting by specific criteria.

bio-flow-cytometry-bead-normalization

GPTomics/bioSkills

Bead-based normalization for CyTOF and high-parameter flow cytometry. Covers EQ bead normalization, signal drift correction, and batch normalization. Use when correcting instrument drift in CyTOF or harmonizing data across batches.

bio-flow-cytometry-clustering-phenotyping

GPTomics/bioSkills

Unsupervised clustering and cell type identification for flow/mass cytometry. Covers FlowSOM, Phenograph, and CATALYST workflows. Use when discovering cell populations in high-dimensional cytometry data without predefined gates.

bio-flow-cytometry-compensation-transformation

GPTomics/bioSkills

Spillover compensation and data transformation for flow cytometry. Covers compensation matrix calculation, application, and biexponential/arcsinh transforms. Use when correcting spectral overlap between fluorophores or transforming data for analysis.

bio-flow-cytometry-cytometry-qc

GPTomics/bioSkills

Comprehensive quality control for flow cytometry and CyTOF data. Covers flow rate stability, signal drift, margin events, dead cell exclusion, and batch QC. Use when assessing acquisition quality or identifying problematic samples before analysis.

bio-flow-cytometry-differential-analysis

GPTomics/bioSkills

Differential abundance and state analysis for cytometry data. Compare cell populations between conditions using statistical methods. Use when testing for significant changes in cell frequencies or marker expression between groups.

bio-flow-cytometry-doublet-detection

GPTomics/bioSkills

Detect and remove doublets from flow and mass cytometry data. Covers FSC/SSC gating and computational doublet detection methods. Use when filtering out cell aggregates before clustering or quantitative analysis.

bio-flow-cytometry-fcs-handling

GPTomics/bioSkills

Read and manipulate Flow Cytometry Standard (FCS) files. Covers loading data, accessing parameters, and basic data exploration. Use when loading and inspecting flow or mass cytometry data before preprocessing.

bio-flow-cytometry-gating-analysis

GPTomics/bioSkills

Manual and automated gating for defining cell populations in flow cytometry. Covers rectangular, polygon, and data-driven gates. Use when identifying cell populations through hierarchical gating strategies.

bio-format-conversion

GPTomics/bioSkills

Convert between sequence file formats (FASTA, FASTQ, GenBank, EMBL) using Biopython Bio.SeqIO. Use when changing file formats or preparing data for different tools.

bio-fragment-analysis

GPTomics/bioSkills

Analyzes cfDNA fragment size distributions and fragmentomics features using FinaleToolkit or Griffin. Extracts nucleosome positioning patterns, fragment ratios, and DELFI-style fragmentation profiles for cancer detection. Use when leveraging fragment patterns for tumor detection or tissue-of-origin analysis.

bio-gatk-variant-calling

GPTomics/bioSkills

Variant calling with GATK HaplotypeCaller following best practices. Covers germline SNP/indel calling, GVCF workflow for cohorts, joint genotyping, and variant quality score recalibration (VQSR). Use when calling variants with GATK HaplotypeCaller.

bio-gene-regulatory-networks-coexpression-networks

GPTomics/bioSkills

Build weighted gene co-expression networks to identify modules of co-regulated genes and relate them to phenotypes using WGCNA and CEMiTool. Detects hub genes and module-trait relationships from bulk or single-cell expression data. Use when finding co-expression modules, identifying hub genes, or relating gene networks to clinical or experimental variables.